Digoxigenin 4455556 222609873 2008-06-30T06:49:58Z Thijs!bot 1392310 robot Adding: [[fr:Digoxygénine]] [[Image:Digoxigenin acsv.svg|right|thumb|200px|chemical structure of digoxigenin]] '''Digoxigenin''' (DIG) is a [[steroid]] found exclusively in the flowers and leaves of the plants ''[[Digitalis purpurea]]'' and ''[[Digitalis lanata]]''. == Use in biotechnology == Its small size and relative ease with which it can be attached to biological molecules, along with the availability of [[antibody|antibodies]] in order to target it, makes it a useful tool in biochemistry. Along with [[fluorescein]], it has become the standard [[immunohistochemistry|immunohistochemical]] marker for [[in situ hybridisation]]{{Fact|date=June 2007}}. In this case it is conjugated to a single species of RNA [[nucleotide]] triphosphate (typically [[Uridine]]), which is then incorporated into the riboprobe as it is synthesised. It may also be conjugated with sugars, allowing the incorporation of specific sugars into [[glycoproteins]] to be imaged in a similar way. == References == *Hauptmann, G.; Gerster, T. (1994). "Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos." ''Trends Genet.'' '''10''' (8): 266. PMID 7940754. == See also == *[[immunostaining]] [[Category:Cardenolides]] [[de:Digoxigenin]] [[fr:Digoxygénine]] [[ja:ジゴキシゲニン]]