Digoxigenin
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2008-06-30T06:49:58Z
Thijs!bot
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robot Adding: [[fr:Digoxygénine]]
[[Image:Digoxigenin acsv.svg|right|thumb|200px|chemical structure of digoxigenin]]
'''Digoxigenin''' (DIG) is a [[steroid]] found exclusively in the flowers and leaves of the plants ''[[Digitalis purpurea]]'' and ''[[Digitalis lanata]]''.
== Use in biotechnology ==
Its small size and relative ease with which it can be attached to biological molecules, along with the availability of [[antibody|antibodies]] in order to target it, makes it a useful tool in biochemistry. Along with [[fluorescein]], it has become the standard [[immunohistochemistry|immunohistochemical]] marker for [[in situ hybridisation]]{{Fact|date=June 2007}}. In this case it is conjugated to a single species of RNA [[nucleotide]] triphosphate (typically [[Uridine]]), which is then incorporated into the riboprobe as it is synthesised.
It may also be conjugated with sugars, allowing the incorporation of specific sugars into [[glycoproteins]] to be imaged in a similar way.
== References ==
*Hauptmann, G.; Gerster, T. (1994). "Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos." ''Trends Genet.'' '''10''' (8): 266. PMID 7940754.
== See also ==
*[[immunostaining]]
[[Category:Cardenolides]]
[[de:Digoxigenin]]
[[fr:Digoxygénine]]
[[ja:ジゴキシゲニン]]