Integrin
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2008-06-14T04:10:58Z
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'''Integrins''' are [[cell surface receptor]]s that interact with the [[extracellular matrix]] (ECM) and mediate various [[cell signaling|intracellular signals]]. They define cellular shape, mobility, and regulate the [[cell cycle]]. These [[integral membrane protein]]s are attached to the cellular [[plasma membrane]] through a single [[transmembrane domain|transmembrane helix]].
Integrin plays a role in the attachment of cells to other cells, and also plays a role in the attachment of a cell to the material part of a tissue that is not part of any cell (the [[extracellular matrix]]). Besides the attachment role, integrin also plays a role in [[signal transduction]], a process by which a cell transforms one kind of signal or stimulus into another.
The integrins are unusual membrane proteins because the signals they convert travel in both outside-in: transducing information from the ECM to the cell, and inside-out: "revealing" the status of the cell to the extracellular world. This allows cells to make rapid and flexible responses (see the example of platelets, in the '''Function''' section). It is more common for cells to make new receptors on their surfaces, or remove them if they need to alter their ability to respond to the environment.
There are many types of integrin, and many cells have multiple types on their surface. Integrins are of vital importance to all [[animals]] and have been found in all animals tested, from [[sponges]] to [[mammals]]. Integrins have been extensively studied in humans.
Other types of [[protein]] that play a role in cell-cell and cell-matrix interaction and communication are [[cadherin]]s, [[Cell adhesion molecule|CAM]]s and [[selectin]]s.
==Structure==
Integrins are [[obligate]] [[heterodimer]]s containing two distinct chains, called the α (alpha) and β (beta) subunits. In [[mammal]]s, 19 α and 8 β subunits have been characterized, whereas the ''[[Drosophila]]'' and ''[[Caenorhabditis]]'' [[genome]]s encode only five α and two β subunits.<ref name=hump>{{cite journal | author= Humphries M.J. | title= Integrin structure | journal=Biochem. Soc. Trans. | volume=28 | issue=4 |pages= 311–339 | year=2000 | pmid= 10961914 | doi = 10.1042/0300-5127:0280311 <!--Retrieved from CrossRef by DOI bot-->}}</ref>
* Alpha: {{Gene|ITGA1}} ([[CD49a]]), {{Gene|ITGA2}} ([[CD49b]]), {{Gene|ITGA2B}} ([[ITGA2B|CD41]]), {{Gene|ITGA3}} ([[CD49c]]), {{Gene|ITGA4}} ([[CD49d]]), {{Gene|ITGA5}} ([[ITGA5|CD49e]]), {{Gene|ITGA6}} ([[CD49f]]), {{Gene|ITGA7}}, {{Gene|ITGA8}}, {{Gene|ITGA9}}, {{Gene|ITGA10}}, {{Gene|ITGA11}}, {{Gene|ITGAD}} (CD11d), {{Gene|ITGAE}} ([[CD103]]), {{Gene|ITGAL}} ([[CD11a]]), {{Gene|ITGAM}} ([[Integrin alpha M|CD11b]]), {{Gene|ITGAV}} ([[CD51]]), {{Gene|ITGAW}}, {{Gene|ITGAX}} ([[CD11c]])
* Beta: {{Gene|ITGB1}} ([[CD29]]), {{Gene|ITGB2}} ([[CD18]]), {{Gene|ITGB3}} ([[CD61]]), {{Gene|ITGB4}} (CD104), {{Gene|ITGB5}}, {{Gene|ITGB6}}, {{Gene|ITGB7}}, {{Gene|ITGB8}}
In addition, variants of some of the subunits are formed by differential splicing, for example 4 variants of the beta-1 subunit exist. Through different combinations of these alpha and beta subunits, some 24 unique integrins are generated, although the number varies according to different studies. <ref name="hynes1">{{cite journal |author=Hynes R |title=Integrins: bidirectional, allosteric signaling machines |journal=Cell |volume=110 |issue=6 |pages=673–87 |year=2002 |pmid=12297042 | doi = 10.1016/S0092-8674(02)00971-6 <!--Retrieved from CrossRef by DOI bot-->}}</ref>
Integrin subunits span the [[plasma membrane]] and in general have very short cytoplasmic domains of about 40-70 [[amino acid]]s. The exception is the beta-4 subunit which has a cytoplasmic domain of 1088 amino acids, one of the largest known cytoplasmic domains of any membrane protein. Outside the cell [[plasma membrane]], the alpha and beta chains lie close together along a length of about 23[[Nanometre|nm]], the final 5nm [[N-terminus|N-termini]] of each chain form a [[ligand (biochemistry)|ligand-binding]] region for the ECM, or [[extracellular matrix]].
The [[molecular mass]] of the integrin subunits can vary from 90 [[atomic mass unit|kDa]] to 160 kDa. β subunits have four [[cysteine]]-rich repeated sequences. Both α and β subunits bind several [[divalent]] [[cation]]s. The role of the α cations is unknown, but they may stabilize the folds of the protein. The β cations are more interesting: they are directly involved in coordinating at least some of the [[ligand (biochemistry)|ligands]] that integrins bind.
There are various ways of categorizing the integrins. For example, a subset of the α chains has an additional structural element (or "domain") inserted toward their [[N-terminal]], the so called alpha-A domain (because it has a similar structure to the A-domains found in the protein [[von Willebrand factor]]: it is also termed the α-I domain). Integrins carrying this domain either bind to [[collagen]]s (e.g. integrins α1 β1, and α2 β1), or act as [[Cell (biology)|cell]]-cell adhesion molecules (integrins of the β2 family). This α-I domain is the binding site for ligands of such integrins. Those integrins that don't carry this inserted domain, also have an A-domain in their ligand binding site, but ''this'' A-domain is found on the β subunit.
In both cases, the A-domains carry up to three divalent cation binding sites. One is permanently occupied in physiological [[concentration]]s of divalent cations, and carries either a calcium or magnesium ion, the principal divalent cations in blood at median concentrations of 1.4 mM (calcium) and 0.8 mM (magnesium). The other two sites become occupied by cations when ligands bind - at least for those ligands involving an acidic amino acid in their interaction sites. An acidic amino acid features in the integrin-interaction site of many ECM proteins, for example, as part of the amino acid sequence [[Arginine]]-[[Glycine]]-[[Aspartic acid]] ("RGD" in the one-letter aminoacid code).
===High resolution structure===
Despite many years of effort, discovering the high resolution structure of integrins proved to be challenging: membrane proteins are classically difficult to purify, and integrins are also large, complex and linked to many sugar trees ("highly [[glycosylation|glycosylated]]"). Low resolution images of detergent extracts of intact integrin GPIIbIIIa, obtained using electron microscopy, and even data from indirect techniques, investigating the solution properties of integrins using ultracentrifugation and light scattering, were combined with fragmentary high resolution crystallographic or NMR data from single or paired domains of single integrin chains, and molecular models postulated for the rest of the chains.
Despite these wide-ranging efforts, the [[X-ray]] crystal structure obtained for the complete extracellular region of one integrin, αvβ3 was a surprise.<ref>{{cite journal |author=Xiong JP | title=Crystal structure of the extracellular segment of integrin αvβ3 |journal=Science |volume=294 |issue=5541 |pages=339–345 |year=2001 |pmid=11546839 | doi = 10.1126/science.1064535 <!--Retrieved from CrossRef by DOI bot-->}}</ref>
It showed the molecule to be folded into an inverted V-shape which brings the ligand-binding sites close to the cell membrane. Perhaps more importantly, the crystal structure was also obtained for the same integrin bound to a small ligand containing the RGD-sequence, the drug [[cilengitide]].<ref>{{cite journal |author=Smith J |title=Cilengitide Merck |journal=Curr Opin Investig Drugs |volume=4 |issue=6 |pages=741–5 |year=2003 |pmid=12901235}}</ref> As detailed above, this finally revealed why divalent cations (in the A-domains) are critical for RGD-ligand binding to integrins. The interaction with such sequences is believed to be a primary switch by which ECM exerts its effects on cell behaviour.
The structure poses many questions, especially regarding ligand binding and signal transduction. The ligand binding site is directed towards the C-terminal of the integrin, the region where the molecule emerges from the cell membrane. If it emerges [[orthogonality|orthogonally]] from the membrane, the ligand binding site would apparently be obstructed, especially as integrin ligands are typically massive, and well cross-linked components of the ECM. In fact, little is known about the angle which membrane proteins subtend to the plane of the membrane - it is a problem difficult to address with available technologies. The default assumption is that they emerge rather like little lollipops - the evidence for this sweet supposition is noticeable by its absence. The integrin structure has drawn attention to this problem, which may have implications for how membrane proteins work.
Although the crystal structure changed surprisingly little after binding to cilengitide, the current hypothesis is that integrin function involves changes in shape to move the ligand binding site into a more accessible position away from the cell surface, and this shape change also triggers intracellular signaling. And there is a wide body of cell biological and biochemical literature that supports this view. Perhaps the most convincing evidence involves the use of [[antibody|antibodies]] that only recognize integrins when they have bound to their ligands, or are activated. As the "footprint" that an antibody makes on its binding target is roughly a circle about 3 nm in diameter, the resolution of this technique is low. Nevertheless, these so-called LIBS (Ligand-Induced-Binding-Sites) antibodies unequivocally show that dramatic changes in integrin shape routinely occur.
==Function==
Two main functions of integrins are:
*Attachment of the cell to the ECM.
*Signal transduction from the ECM to the cell.
However, they are also involved in a wide range of other biological activities. These include: binding of viruses, including [[adenovirus]], Echo viruses, Hanta viruses and [[foot and mouth disease]] viruses, to cells; immune patrolling. Cell migration.
A very prominent function of the integrins is seen in the molecule GPIIbIIIa, an integrin on the surface of blood platelets (thrombocytes) responsible for cross-linking platelets in fibrin within a developing blood clot. This switches its adhesiveness for fibrin/fibrinogen from being non-adhesive to being intensely sticky, in a fast and precisely controlled manner. As such it provides a thought-model for how many integrins are believed to be regulated. As you may have noted, although blood is normally very rich in platelets, we do not spontaneously clot. This is clearly good news. On the other side, and equally positively, even minor wounds are rapidly blocked by the mass of fibrin, platelets and erythrocytes in a blood clot. A primary event in clot formation is the binding of platelets to exposed collagen in the wound site, which leads to their "activation", and a clotting cascade. Among the many molecular events during activation, is the switching of GPIIbIIIa integrin from a quiescent state, unable to bind to fibrinogen/fibrin, to an active state, able to bind strongly to fibrinogen/fibrin. This is a remarkable event: first it involves all the GPIIbIIIa on a single platelet (some 50000 molecules), second it is completed within 5 seconds, third, it increases the affinity of the integrin concerned over several orders of magnitude. Fourth, it involves wide spread changes in the molecular structure of the GPIIbIIIa molecule, as resolved by LIBS antibodies, which gain the ability to bind GPIIbIIIa only following activation of the platelets. Finally, it is intensely locallized to the precise region of the damage, be it a couple of square micrometres, or the results of falling off a mountain bike at high speed.
=== Attachment of cell to the ECM===
Integrins couple the ECM outside a cell to the [[cytoskeleton]] (in particular the [[microfilaments]]) inside the cell. Which ligand in the ECM the integrin can bind to is mainly decided by which α and β subunits the integrin is made of. Among the [[ligand]]s of integrins are [[fibronectin]], [[vitronectin]], [[collagen]], and [[laminin]]. The connection between the cell and the ECM may help the cell to endure pulling forces without being ripped out of the ECM. The ability of a cell to create this kind of bond is also of vital importance in [[ontogeny]].
Cell attachment to the ECM is a basic requirement to build a multicellular organism. Integrins are not simply hooks, but give the cell critical signals about the nature of its surroundings. Together with signals arising from receptors for soluble growth factors like [[VEGF]], [[Epidermal growth factor|EGF]] and many others, they enforce a cellular decision on what biological action to take, be it attachment, movement, death, or differentiation. Thus integrins lie at the heart, both literally and figuratively, of many cellular biological processes. The attachment of the cell takes place through formation of [[cell adhesion]] complexes, which consist of integrins and many cytoplasmic proteins which include [[Talin (protein)|talin]], [[vinculin]], paxillin and alpha-actinin. These act by regulating [[kinase]]s like FAK ([[focal adhesion kinase]]) and Src kinase family members to phosphorylate substrates such as p130CAS thereby recruiting signaling adaptors such as Crk. These adhesion complexes attach to the actin cytoskeleton. The integrins thus serve to link across the plasma membrane two networks: the extracellular ECM and the intracellular actin filamentous system.
One of the most important functions of surface integrins is their role in [[cell migration]]. Cells adhere to a [[Substrate (biology)|substrate]] through their integrins. During movement, the cell makes new attachments to the substrate at its front and concurrently releases those at its rear. When released from the substrate, integrin molecules are taken back into the cell by [[endocytosis]]; they are transported through the cell to its front by the [[endocytic cycle]] where they are added back to the surface. In this way they are cycled for reuse, enabling the cell to make fresh attachments at its leading front.
===Signal transduction===
Integrins play an important role in cell signaling. Connection with ECM molecules can cause a signal to be relayed into the cell through protein [[kinase]]s that are indirectly and temporarily connected with the intracellular end of the integrin molecule, likely following shape changes directly stimulated by ECM binding.
The signals the cell receives through the integrin can have relation to:
*[[cell growth]]
*[[cell division]]
*cell survival
*[[cellular differentiation]]
*[[apoptosis|apoptosis (programmed cell death)]]
==Vertebrate integrins==
The following are some of the integrins found in vertebrates:
{| class="wikitable"
| '''Name''' || '''Synonyms''' || '''Distribution''' || '''Ligands'''
|-
| '''α<sub>1</sub>β<sub>1</sub>''' || || Many || [[Collagen]]s, [[laminin]]s.<ref name=lodish> Molecular cell biology. Lodish, Harvey F. 5. ed. : - New York : W. H. Freeman and Co., 2003, 973 s. b ill. ISBN 0-7167-4366-3</ref>
|-
| '''α<sub>2</sub>β<sub>1</sub>''' || || Many || Collagens, laminins<ref name=lodish/>
|-
| '''[[VLA-4|α<sub>4</sub>β<sub>1</sub>]]''' || VLA-4<ref name=lodish/> || [[Hematopoietic]] cells || [[Fibronectin]], [[VCAM-1]]<ref name=lodish/>
|-
| '''[[alpha-5 beta-1|α<sub>5</sub>β<sub>1</sub>]]''' || fibronectin receptor || widespread || fibronectin<ref name=lodish/> and [[proteinase]]s
|-
| '''alpha-6 beta-1|α<sub>6</sub>β<sub>1</sub>''' || laminin receptor || widespread || [[matrix macromolecules]] laminins
|-
| '''[[LFA-1|α<sub>L</sub>β<sub>2</sub>]]''' || LFA-1<ref name=lodish/> || [[T-lymphocyte]]s || [[ICAM-1]], [[ICAM-2]]<ref name=lodish/>
|-
| '''[[Mac-1|α<sub>M</sub>β<sub>2</sub>]]''' || Mac-1, CR3<ref name=lodish/> || [[Neutrophil]]s and [[monocyte]]s || [[Serum]] proteins, ICAM-1<ref name=lodish/>
|-
| '''α<sub>IIb</sub>β<sub>3</sub>''' || || [[Platelet]]s<ref name=lodish/> || fibrinogen, fibronectin<ref name=lodish/>
|-
| '''[[alpha-v beta-3|α<sub>V</sub>β<sub>3</sub>]]''' || vitronectin receptor<ref name=VR/> || activated endothelial cells, melanoma, glioblastoma|| [[vitronectin]] <ref name=VR> [http://www.ncbi.nlm.nih.gov/pubmed/10037797 PMID: 10037797]</ref>, fibronectin, fibrinogen, osteopontin, Cyr61
|-
| '''[[alpha-v beta-5|α<sub>V</sub>β<sub>5</sub>]]''' || || widespread, esp. fibroblasts, epithelial cells || [[vitronectin]] and adenovirus
|-
| '''alpha-v beta-6|α<sub>V</sub>β<sub>6</sub>''' || || proliferating epithelia, esp. lung and liver || [[fibronectin]]; TGFβ1+3
|-
| '''α<sub>6</sub>β<sub>4</sub>''' || || [[Epithelial]] cells<ref name=lodish/> || [[Laminin]]<ref name=lodish/>
|}
Beta1 integrins interact with many alpha integrin chains.
Gene knockouts of integrins in mice are not always lethal. It proves that during embryonal development, one integrin may substitute its function for another, to allow survival.
Some integrins are on the cell surface in an inactive state, and can be rapidly primed, or put into a state capable of binding their ligands, by cytokines. Integrins can assume several different well defined shapes, or "conformational states". Once primed, the conformational state changes to stimulate ligand binding which then activates the receptors, also by inducing a shape change, to trigger outside-in signal transduction.
==Additional images==
<gallery>
Image:Integrin.png|Integrin (captions in German)
</gallery>
== References ==
<div class="references-small">
<references/>
</div>
==External links==
* [http://macromoleculeinsights.com/integrin.php The Integrin Protein]
* {{MeshName|Integrins}}
{{Signaling proteins}}
{{Immune receptors}}
{{Cell adhesion molecules}}
[[Category:Transmembrane proteins]]
[[Category:Cell adhesion proteins]]
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