Phenol extraction
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2007-11-21T10:59:09Z
Malcolma
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cat
'''Phenol extraction''' is a method used to purify a [[DNA]] sample.
In this method, a mixture of TE (or [[Tris]]-[[Ethylenediaminetetraacetic acid]]) and [[phenol]] is combined with an equal volume of an aqueous [[DNA]] sample. After agitation and centrifugal separation, the aqueous layer is extracted, and further processed with [[diethyl ether|ether]]. Then the DNA is concentrated by [[ethanol precipitation]].
==See also==
*[[Phenol-chloroform extraction]]
==References==
*http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/phenol.html
[[Category:Molecular biology]]
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