Polysorbate 20 3752212 215514576 2008-05-28T15:17:15Z DOI bot 6652755 Citation maintenance. You can [[WP:DOI|use this bot]] yourself! Please [[User:DOI_bot/bugs|report any bugs]]. {{Chembox new | Name = Polysorbate 20 | ImageFile = Polysorbate 20.png | ImageSize = 250px | ImageName = Polysorbate 20 | IUPACName = Polyoxyethylene (20) sorbitan monolaurate | OtherNames = Polysorbate 20<br />PEG(20)sorbitan monolaurate | Section1 = {{Chembox Identifiers | CASNo = 9005-64-5 | RTECS = TR7400000 }} | Section2 = {{Chembox Properties | Formula = C<sub>58</sub>H<sub>114</sub>O<sub>26</sub> | MolarMass = 1227.54 g/mol | Appearance = Clear, yellow to yellow-green viscous liquid. | Density = 1.1 g/mL (approximate) <!-- Per MSDS - this MUST be known, somewhere! --> | Solubility = | MeltingPt = | BoilingPt = > 100 °C }}<!-- | [[Hydrophilic-lipophilic balance|HLB]] | 16.7 |- --> | Section7 = {{Chembox Hazards | ExternalMSDS = [http://www.caymanchem.com/msdss/583151m.pdf External MSDS] | MainHazards = Irritant | NFPA-H = 1 | NFPA-F = | NFPA-R = | FlashPt = 110 °C }} }} '''Polysorbate 20''' (commercially also known as '''Tween 20''') is a [[polysorbate]] [[surfactant]] whose stability and relative non-toxicity allows it to be used as a [[detergent]] and [[emulsifier]] in a number of domestic, scientific, and pharmacological applications. It is a [[polyethylene glycol|polyoxyethylene]] derivative of sorbitan monolaurate, and is distinguished from the other members in the Tween range by the length of the polyoxyethylene chain and the fatty acid ester moiety. The commercial product contains a range of chemical species.<ref>{{cite journal | author = Ayorinde FO, Gelain SV, Johnson JH Jr, Wan LW. | year = 2000 | title = Analysis of some commercial polysorbate formulations using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry | journal = Rapid Communications in Mass Spectrometry | volume = 14 | issue = 22 | pages = 2116&ndash;2124 | pmid = 11114018 | doi = 10.1002/1097-0231(20001130)14:22<2116::AID-RCM142>3.0.CO;2-1 | doilabel = 10.1002/1097-0231(20001130)14:22&#60;2116::AID-RCM142&#62;3.0.CO;2-1 }}</ref> ==Food applications== Tween is used as a [[wetting agent]] in flavored mouth drops such as Ice Drops, helping to provide the spreading feeling of the other ingredients like SD alcohol and mint flavor. The [[World Health Organization]] has suggested acceptable daily intake limits of 0-25 mg of polyoxyethylene sorbitan esters per kg body weight.<ref>{{cite web | url = http://www.inchem.org/documents/jecfa/jecmono/v05je47.htm | title = Toxicological evaluation of some food additives including anticaking agents, antimicrobials, antioxidants, emulsifiers and thickening agents | author = Joint FAO/WHO Expert Committee on Food Additives | date = 1974 | work = WHO Food Additives Series No. 5 | publisher = [[World Health Organization]] }}</ref> ==Biological applications== In the biological sciences, Tween 20 has a broad range of applications. For example, it is used: * to stabilize [[purified protein derivative]] (PPD) solution used in skin testing for [[tuberculosis]] exposure * as a blocking agent in [[immunoassay]]s, such as [[Western blot]]s and [[ELISA]]s. * as a solubilizing agent of [[Cell membrane|membrane]] proteins * for [[lysis|lysing]] mammalian cells, at a concentration of 0.05% to 0.5% v/v. * can be dissolved in [[Tris-Buffered Saline]] or [[Phosphate buffered saline]] at dilutions of 0.05% to 0.5% v/v and used as a washing solution for immunoassays. ==Pharmaceutical applications== Tween is used as in pharmaceutical applications to stabilize [[emulsion]]s and [[suspension (chemistry)|suspension]]s. ==Industrial and domestic applications== The [[Clorox]]-made cleaning solution used by the [[iRobot]] [[Scooba]] floor-washing robot lists Tween 20 as one of its major ingredients on its [[MSDS]]. ==Data== Tween 20's [[critical micelle concentration|cmc]] is 8.04&times;10<sup>-5</sup> M at 21°C.<ref name="ChunheeKim">{{cite journal |author = Chunhee Kim, You-Lo Hsieh |journal = Colloids and Surfaces A |title = Wetting and absorbency of nonionic surfactant solutions on cotton fabrics |year = 2001 |volume = 187 |pages = 385–397 |doi = 10.1016/S0927-7757(01)00653-7 }}</ref> ==References== {{reflist}} ==See also== * [[Polysorbate 80]] [[Category:Surfactants]] [[de:Polysorbat 20]] [[hu:Polioxietilén(20)-szorbitán-laurát]] [[nl:Polysorbaat 20]]