Sodium orthovanadate 3482079 210300396 2008-05-05T11:18:03Z Axiosaurus 3399137 added a little content re structure and corrected the IUPAC name {{Chembox new | IUPACName = Sodium vanadate(V) | OtherNames = sodium vanadate (V),<br />sodium vanadium oxide | Section1 = {{Chembox Identifiers | CASNo = 13721-39-6 | RTECS = YW1120000 }} | Section2 = {{Chembox Properties | Formula = [[Sodium|Na]]<sub>3</sub>[[Vanadium|V]][[Oxygen|O]]<sub>4</sub> | MolarMass = 183.91 g/mol | Appearance = white powder | Density = 2.16 g/cm³, solid | Solubility = soluble }}<!-- | [[Melting range]] | 850-866°C |- --> | Section7 = {{Chembox Hazards | ExternalMSDS = | MainHazards = Harmful. | NFPA-H = 2 | NFPA-F = 1 | NFPA-R = | FlashPt = Non-flammable }} }} '''Sodium orthovanadate''' is the chemical compound Na<sub>3</sub>VO<sub>4</sub> and contains the tetrahedral VO<sub>4</sub><sup>3−</sup>.<ref>{{Greenwood&Earnshaw}}</ref> It is an [[Enzyme inhibitor|inhibitor]] of [[protein tyrosine phosphatase]]s, [[alkaline phosphatase]]s and a number of [[ATPase]]s, most likely acting as a [[phosphate]] analogue. The VO<sub>4</sub><sup>3-</sup> [[ion]] binds irreversibly to the active sites of most [[protein tyrosine phosphatase]]s. It is often added to [[buffer solution]]s that are used in [[protein analysis]] in [[molecular biology]]. The aim is to preserve the [[phosphorylation]] of proteins of interest by inhibiting endogenous phosphatases present in [[cell lysate]] mixture.<!-- and to prevent the degradation of [[protein]]s of interest by endogenous proteases present in [[cell lysate]] mixture. DOUBTFUL--> It is commonly used at a final [[concentration]] of 10 - 100mM. It is [[toxic]] when inhaled, swallowed or in contact with skin. ==References== {{reflist}} :Swarup, G. et al. Biochem. Biophys. Res. Comm. 107: 1104-1109 (1982). ==See also== *[[Sodium metavanadate]] [[Category:Inorganic compounds]] [[Category:Sodium compounds]] [[Category:Vanadates]]