Triton X-100 3580779 221186215 2008-06-23T12:28:40Z DumZiBoT 6085301 robot Adding: [[it:Triton X-100]] {{Chembox new | Name = Triton X-100 | ImageFile = Triton X-100.png | ImageFile1 = | IUPACName = | OtherNames = polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether, <br/> octyl phenol ethoxylate, <br/> polyoxyethylene octyl phenyl ether, <br/> 4-octylphenol polyethoxylate, <br/> Mono 30, <br/> TX-100, <br/> t-octylphenoxypolyethoxyethanol, <br/> Octoxynol-9 | Section1 = {{Chembox Identifiers | SMILES = | CASNo = 9002-93-1 | PubChem = }} | Section2 = {{Chembox Properties | Formula = {{chem|C|14|H|22|O(C|2|H|4|O)|n}} (n = 9-10) | MolarMass = | Appearance = viscous colourless liquid | Density = 1.07 g/cm<sup>3</sup> | MeltingPt = 6°C | BoilingPt = >200°C | Solubility = Soluble | VaporPressure = <1mmHg at 20°C }} | Section4 = {{Chembox Thermochemistry }} | Section7 = {{Chembox Hazards | ExternalMSDS = [http://physchem.ox.ac.uk/MSDS/TR/triton_X-100.html MSDS] | NFPA-H = | NFPA-F = | NFPA-R = | FlashPt = 251 °C }} }} '''Triton X-100''' ({{chem|C|14|H|22|O(C|2|H|4|O)|n}}) is a [[nonionic]] [[surfactant]] which has a [[hydrophilic]] [[polyethylene oxide]] group (on average it has 9.5 ethylene oxide units) and a [[hydrocarbon]] [[lipophilic]] or [[hydrophobic]] group. The hydrocarbon group is a 4-(1,1,3,3-tetramethylbutyl)-[[phenyl]] group.<ref>http://www.sigmaaldrich.com/sigma/product%20information%20sheet/t8532pis.pdf</ref> It is related to the [[Pluronic]] range of [[detergent]]s marketed by [[BASF]]. The pluronics are triblock copolymers of [[ethylene oxide]] and [[propylene oxide]]. The part formed from ethylene oxide is more hydrophilic than the part from propylene oxide. It is very viscous at room temperature and is thus easiest to use after being gently warmed. Triton X-100 is a registered trademark of [[Union Carbide]] and was purchased from Rohm & Haas Co. ==Uses== *Commonly used [[detergent]] in [[biochemistry]] laboratories. *It can be used to permeabilize eukaryotic cell membranes *It is used in conjunction with [[zwitterion]]ic detergents such as [[CHAPS detergent|CHAPS]] to solubilize [[membrane protein]]s in their native state. *It is used in almost every type of liquid, paste, and powdered cleaning compound, ranging from heavy-duty industrial products to gentle detergents. *Can be used in [[DNA extraction]] as part of the [[lysis buffer]] (usually in a 5% solution in alkaline lysis buffer). *It can be used for cell permeabilization for [[Immunostaining]] (usually in concentration of 0,1-0,5% in TBS or PBS Buffer). *Popular ingredient in home made [[vinyl record]] cleaning fluids together with distilled water and isopropanol. ==References== <references/> ==External links== * [http://www.dow.com/surfactants/products/octyl.htm List of different available Triton x (other octylphenol ethoxylates) detergents] [[Category:Surfactants]] [[Category:Aromatic compounds]] {{aromatic-stub}} [[de:Octoxinol-9]] [[fr:Triton X-100]] [[it:Triton X-100]] [[pl:Triton X-100]]